囊性纖維化PCR芯片可用于分析在囊性纖維化（CF）差異表達或與囊性纖維化跨膜電導調節（CFTR）相互作用的84個關鍵基因的表達。CFTR的突變會引起消化系統和呼吸系統的失調，CF患者會有不同程度的支氣管粘液增厚，以及慢性肺炎導致的肺纖維化和肺功能衰竭目前的研究集中在患者逐漸惡化的CF肺部病變，通過治療可以延長CF患者40年的平均壽命，但導致疾病及其癥狀的分子機制尚未發掘。這一芯片包含了離子轉運蛋白基因、參與免疫或炎癥反應的基因、可能與CFTR功能有關的基因。通過實時定量PCR的方法，研究者即能夠利用該芯片簡單可靠地同時檢測CFTR分子機制相關基因的表達。
Modifier Genes: ADIPOR2, ADRB2, EDNRA, GCLC, GSTM1 (MGST1), IFRD1, IL8, MBL2, MSRA, NOS3 (eNOS), SCNN1B, SCNN1G, SERPINA1, TCF7L2, TGFB1, TNF, TNFRSF1A. Genes Interacting with CFTR: AHSA1, CANX, CFTR, DNAJA1, DNAJC5, EZR, GOPC, HSP90AA1, HSPA4 (HSP70), HSPA8, NME1 (NM23A), PDZK1, PPP2R4, PRKAA1, PRKAA2, SLC26A3, SLC9A3R1, SLC9A3R2, SNAP23, STX1A, STX8, TGFB1, TJP1, VCP. Neutrophil Chemotaxis: CCL2 (MCP1), CXCR2 (IL8RB), EDN1, EDNRA, IL1B, IL8, ITGB2. Inflammatory Response: ACE, ADRB2, ALOX12B, CCL2 (MCP1), CXCL1, CXCL2, DEFB1, EDNRA, IL10, IL1B, IL6, IL8, ITGB2, MBL2, NFKB1, PLA2G5, PTGS2 (COX2), S100A8, SERPINA1, TGFB1, TLR2, TLR4, TLR5, TNF, TNFRSF11A (RANK), TNFRSF1A. Immune Response: Innate: CCL2 (MCP1), CLU, DEFB1, ICAM1, LCN2 (NGAL), MAPK1 (ERK2), MBL2, NFKB1, NFKBIA (I?Ba), PRKCE, TGFB1, TLR2, TLR4, TLR5, TNFSF10 (TRAIL). Adaptive: CLU, FAS (TNFRSF6), ICAM1, IL10, IL1B, IL7R, MBL2, TGFB1, TLR4, TNF, TNFRSF11A (RANK). Unfolded Protein Response: CALR, DNAJA1, HSP90AA1, HSPA1A, HSPA4 (HSP70), HSPA8, HSPH1 (HSP105), VCP. Ion Binding & Transport: ADK, KCNE1, NR4A2 (NUR77), SCNN1B, SCNN1G, SLC9A3R1. Oxidative Stress: CLU, DUSP1 (PTPN16), GCLC, IL6, LCN2 (NGAL), MBL2, MSRA, NOS3 (eNOS), PTGS2 (COX2), SFTPB. Other Genes: EPSTI1, IGFBP5, ITGA2, KIT (CD117), MET, PRTN3, SLPI. |
